AGILENT TECHNOLOGIES Seminar
New Levels of Efficiency in your Purification Workflow
Presenter: Dr Andreas Tei
At the Agilent lunch seminar we extend the comprehensive Purification Solutions Portfolio and introduce two new Preparative Fraction Collectors and the enhanced Agilent Automated Purification Software.
The Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector is a highly efficient module for all high capacity purification tasks. The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector combined with the new Agilent Automated Purification Software is the ideal solution that automatically manages high-throughput fraction collection of purified compounds and scale-up workflows from analytical to semi-and preparative scale every day for everyone!
DAISO FINE CHEM Seminar
Necessary Evil: The CIP in Process Scale HPLC
Presenter: Dr Imre Sallay
Insulin, insulin analogues and related diabetes treatment peptide based APIs are the biggest application for silica based HPLC in process scale.
These molecules are prone to self-aggregation and fibrillation rendering the big silica packed columns useless in intolerably short time.
To remove the fibrillated peptide layer from the column unspeakably the silica is treated usually with pH 13 NaOH solution. This latter damaging the silica based stationary phase and again shortening the useful time of the column. Breaking out of this predicament is much needed, but before that we need scientific tools to study the actual situation of the “dirtiness” of the column and the damage suffered by the silica.
ROTACHROM TECHNOLOGIAI Seminar
What is Industrial-scale CPC and How Does it Outperforms Traditional Liquid Chromatography?
Presenter: Dr Laszlo Lorantfy
Centrifugal Partition Chromatography (CPC) is one of the technical realization of liquid-liquid chromatographic, where the liquid stationary phase is immobilized by a strong centrifugal force, coming from a circular motion. A series connected network of extraction cells, which operates as elemental contionous extractors, guarantees the efficiency by the cascade. CPC instruments vary on scale from 50 ml to 25 liter, however all advantages are realized on bigger scale use in industry as it provides a more cost effective and more efficient separation to preparative HPLC. Up to date there were no real available instruments, those could be used for API purification in real situations.
By industrial size we mean process chromatography, the same scale that can be achieved by conventional HPLC DAC columns, with diameter of 150-1200 mm, and flow rate of 1 to 20 liters / minute. So far tt was impossible for the CPC to get a role in API purification without this ability.
In this presentation, we would like to introduce the basics of CPC, and highlight the development steps that provided the pharmaceutical industry the first real industrial CPC, the RotaChrom iCPC. Starting from theoretical basics of the flow simulations. At last, we would like to present applications of our new technology from different examples of API purification in the pharmaceutical industry, compared to preparative HPLC and other techniques, proving that prep HPLC is the past for API purification.
AKZO NOBEL PULP AND PERFORMANCE CHEMICALS AB / KOMASIL Seminar
Latest Stationary Phase Choices for Preparative Chromatography
Presenters: Dr Cecilia Mazza, Dr Mattias Bengtsson, Dr Harald Dibowski
The seminar will focus on the latest developments of Kromasil materials including EternityXT, a stationary phase with organo silane merged into the base silica matrix. EternityXT , with its high chemical and mechanical stability, as well as its scalability, makes it possible for users to work under tough pH conditions and higher temperatures across the various stages of product development including discovery, pilot plant and manufacturing. We will discuss Kromasil EternityXT resistance to high alkaline concentrations for in-place regeneration and share examples of relevant applications.
CHIRAL TECHNOLOGIES EUROPE
20 years of Innovation in Enantiomeric Separations
Presenter: Dr Pilar Franco
Several milestones have represented major step changes at analytical and preparative level in the history of enantiomer separations. DAICEL and CHIRAL TECHNOLOGIES EUROPE have been key players accompanying this evolution with Innovation Products and Local Technical Support over more than two decades.
The commercial introduction of Immobilized polysaccharide-derived Chiral Stationary phases (CSPs) in 2004, the development of innovative screening strategies, the launch of more than 700 product are some of the achievements in the quest of breakthrough solutions. Looking back on more than two decades of perspective in the use of CSPs, this presentation will review the major historical innovation highlights in the field and the ones to come.